Reagents / Amino Acids / Cell Culture Additives
Essential Tools for Cell Culture Workflows
PAN-Biotech’s portfolio of reagents and media supplements supports reliable and reproducible cell culture, buffer preparation, and experimental workflows across research, industrial and clinical applications. Each product is manufactured and tested under strict quality controls to ensure performance, sterility and consistency.
HAT Supplement (50×)
HAT Supplement is a sterile, concentrated solution containing hypoxanthine, aminopterin
and thymidine. It is used for selective cell culture applications, particularly in hybridoma
technology, where it supports the selection of cells utilizing the salvage pathway for
nucleotide synthesis when supplemented to basal media.
HT Supplement (50×)
HT Supplement is a defined, sterile concentrate of hypoxanthine and thymidine without
aminopterin. It is commonly used to support the maintenance and recovery of cells following
HAT selection or in non-selective culture conditions requiring nucleotide precursors.
HEPES Buffer 1 M
HEPES Buffer is a high-capacity buffering solution used to stabilize pH in cell culture media
independently of CO₂ concentration. It is particularly useful for handling steps outside
controlled incubator conditions and for applications requiring stable pH during transport or
manipulation.
Sodium Bicarbonate 7.5 % Solution
Sodium bicarbonate solution is a sterile buffer component used to adjust bicarbonate levels
in cell culture media. It supports physiological pH regulation in CO₂-based incubation systems
and is commonly applied during media preparation and customization.
Sodium Pyruvate 100 mM
Sodium pyruvate is a ready-to-use supplement providing an additional carbon and energy
source for cultured cells. When added to basal media, it supports cellular metabolism and is
frequently used for cell lines with increased energy demands.
ITS Solution I (100×)
ITS Solution I is a sterile, defined supplement containing insulin, transferrin and selenium. It
is designed for use in serum-reduced or serum-free culture systems to support cell growth,
metabolism and viability when added to basal media.
ITS Solution II (100×)
ITS Solution II provides insulin, transferrin and selenium in an alternative formulation suitable
for a broad range of cell culture applications. It is used to enrich basal media under defined
conditions and supports consistent cell performance in reduced-serum environments.
ITS Solution IV (100×)
ITS Solution IV is a defined ITS supplement formulated for use in serum-free or low-serum
culture systems where controlled micronutrient supplementation is required.
Insulin, Human Recombinant 10 mg/ml
Human recombinant insulin is supplied as a sterile solution and is used as a defined growth
and metabolic supplement in cell culture. It supports glucose uptake and anabolic processes
in insulin-responsive cell types and is commonly applied in serum-free formulations.
Pluronic® F-68 10 %
Pluronic® F-68 is a non-ionic surfactant used in cell culture to protect cells from shear stress.
It is particularly applied in suspension cultures and bioreactor systems to improve cell viability
under dynamic conditions.
Sodium Chloride Solution 0.9 %
Sodium chloride 0.9 % is a sterile isotonic saline solution used for general laboratory and cell
handling applications. It supports osmotic balance during dilution, washing and preparation
steps in cell culture workflows.
L-Glutamine 200 mM
L-Glutamine is a sterile, ready-to-use 200 mM stock solution of an essential amino acid
widely required in mammalian and insect cell culture. It provides a key source of carbon
and energy and plays a crucial role in nucleic acid synthesis and overall cell metabolism. Supplementation with L-Glutamine supports optimal cell performance in a wide range of
culture systems.
Stable Glutamine 200 mM (100×)
Stable Glutamine is a 100× concentrated dipeptide (L-alanyl-L-glutamine) solution
designed to overcome the instability of standard glutamine in culture media. This enhanced
formulation provides cells with a sustained glutamine source, reducing degradation and
supporting consistent viability and growth across adherent and suspension cultures.
MEM Amino Acids Solution (50×) w/o L-Glutamine
A concentrated non-glutamine amino acid solution formulated to supply essential amino
acids for cell culture. It supports basic metabolic functions when added to basal media,
particularly when glutamine is managed separately.
MEM Amino Acids Solution (50×)
with L-Glutamine
This concentrated MEM amino acid supplement includes L-Glutamine to provide a comprehensive mix of essential amino acids in one supplement, simplifying media preparation for routine cell culture.
MEM NEAA (100×)
The MEM Non-Essential Amino Acid (NEAA) solution supplies non-essential amino acids to support cell metabolism and relieve intracellular synthesis demands, contributing to improved growth performance.
Gelatine Solution 0.1 % in DPBS
Gelatine Solution 0.1 % in DPBS is a sterile attachment factor supplied in a buffered DPBS base and intended for coating cell culture vessels to improve adhesion of adherent cells. It is commonly used as a surface coating to support attachment of adherent cells in cell culture applications.
Gelatine Solution 2 % in DPBS, w/o Ca & Mg
Gelatine Solution 2 % in DPBS without calcium and magnesium is a sterile coating reagent designed to enhance attachment of adherent cells in culture. The higher concentration of gelatine supports robust cell adhesion for workflows involving endothelial cells, stem cells or other cell types requiring enhanced surface support.
Balanced Salt / Buffered Solutions
Balanced salt solutions are fundamental reagents in cell culture for maintaining
physiological pH and osmolarity during washing, dilution and handling steps. These
sterile solutions provide essential inorganic ions and support consistent, reproducible
performance in laboratory workflows.
Dulbecco’s Phosphate Buffered Saline (DPBS)
DPBS is a sterile buffered salt solution widely used in cell culture for washing, dilution, and handling of cells and tissues while maintaining physiological pH and osmolarity. PAN-Biotech offers DPBS in multiple variants with or without calcium and magnesium to support different application requirements, including gentle cell handling or workflows where divalent cations
must be excluded. The portfolio further includes 10× concentrated formulations for flexible dilution as well as non-sterile variants for applications where sterility is controlled during processing. These DPBS solutions provide consistent ionic conditions and reliable performance across routine and specialized cell culture workflows.
Earle’s Balanced Salt Solution (EBSS)
EBSS provides a physiologically balanced ionic environment for mammalian cell culture,
supporting gentle washing, dilution, and handling of cells. PAN Biotech offers EBSS variants
with or without calcium and magnesium, and with customized sodium bicarbonate
concentrations, enabling flexible adaptation to different cell culture conditions and
experimental needs.
Hank’s Balanced Salt Solution (HBSS)
HBSS is a sterile, buffered salt solution designed to maintain osmotic balance and
physiological pH in cell culture workflows. PAN Biotech provides multiple HBSS variants tailored
to diverse applications, including options with or without calcium and magnesium, with
or without phenol red for visual pH monitoring, and in concentrated 10× format for flexible
preparation.
Gibco’s Balanced Salt Solution (GBSS), w: 2.27 g/L NaHCO₃
GBSS with sodium bicarbonate adds essential buffering capacity and inorganic ions, supporting pH and osmotic balance during cell washing and handling steps in culture workflows.